Karl Edisson Marquez
Scientist
Profile
Scientist with 12 years of extensive industry experience, passionate about leveraging programming languages to transform complex biological data into actionable insights, enabling informed decisions to advance the field of cell therapy.
Address
9095 Sydney Court Suite 10118
San Diego, CA 92122
Contact
Phone
(858) 8420066
Email
karl4marquez@gmail.com
Website
karlmarquez.com
Laboratory Skills
Flow Cytometry

Gene Editing

In-vitro Assays

In-vivo Studies

Cell Culture

Protein Biology

Lab Management

Programming
R

Python

HTML/CSS

Karl Edisson Marquez
Scientist
Experience
2019-Present

Poseida Therapeutics, Inc.

Cell Engineering and Gene Editing of T-cells, managed and worked with matrix teams in discovery and platform development, leading to IND submissions of multiple CAR-T programs targeting BCMA and MUC-1. Advanced the Healthy Donor Program, utilizing cell separation technologies, in-vitro/in-vivo characterization, and correlation/statistical analysis to support Poseida's Allogeneic programs.

2017-2019

ImmunityBio (formerly Nantkwest)

Development and optimization of NK-cell isolations from PBMC and Cord blood sources from small-scale to GMP-grade bioreactors. Demonstrated NK functional cytotoxicity to multiple target cell lines and safety profile to multiple PBMC healthy donors.

2014-2017

Intrexon Corp.

In the nanoparticle field, isolation of exosomes from Bovine milk/cell supernatant, optimizing a combination of exosome purification techniques (ultra-centrifugation, size-exclusion chromatography), verification of isolated exosomes, and high-throughput screening characterization of loaded exosomes in human fibroblasts to measure bioactivity.

2011-2014

Stem Cell Core Facility, UCSD

Human embryonic stem cell culture, provided stem cells to UCSD graduate students by manual/ enzymatic passaging to maintain pluripotent state. Daily operation, maintenance, and calibration of Flow cytometers and sorters, purifying experiment-specific cell subsets, ranging from human immune cells and neurons, to mouse and plants.

Scientific Papers and Patents
2023, ASH

Stacey Cranert, Karl Marquez, et.al. A Tumor-Bearing Murine Xenograft Model As a Bioassay for Assessing CAR-T Product Potency Shows Positive Predictive Value for Clinical Performance

2022, ASGCT

Blair B. Madison, Karl Marquez, et.al. Cas-Clover is a novel high-fidelity nuclease for safe and robust generation of Tscm-enriched allogeneic CAR-T cells

2021, SITC

Hubert Tseng, Karl Marquez, et.al. Memory Phenotype in Allogeneic Anti-BCMA CAR-T Cell Therapy (P-BCMA-ALLO1) Correlates with In Vivo Tumor Control

2019, WIPO

Rohit Dunggal, Karl Marquez, et.al. Mononuclear Derived NK cells. Patent (Pending), US20210009953

Interests
Data Analysis
Statistics
Data Visualization
Programming